The Callus Intection and Isolation of Alkaloid from Datura Metal L

Datura metal L is an important medicinal plant containing the alkaloids like scopalamine(hyoscine),hyoscyamine(atropine) scutellaerin etc .The total alkaloids present in the callus were higher than the amount of alkaloids present in leaves .For the higher production of alkaloids the medium plays avital role.The addition of L-arginine were enhanced the alkaloid production The higher production of alkaloids like scopalamine (Hyoscine) and Hyoscyamine (Atropine) in sodium alignate were conformed by using FTIR – spectra and UV –visible spectra. The realase of alkaloids from the sodium alginate enttrapped cells was higher on 11 day of culture. In the present study indicated that the maximum amount of alkaloids were observered in callus (16.50mg/g d.wt) than leaves (12.37 mg/g d.wt) and seeds (11.5 mg/g dwt). The ms medium supplimented with different concentration of auxin (NAA) and cytokinin (BA) were enhanced maximum number of the shoot buds .Auxin rich medium promoted the rhizogenesis directly from callus.